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Development of a highly sensitive inhibition immunoassay for microcystin-LR [An article from: Analytica Chimica Acta]
Development of a highly sensitive inhibition immunoassay for microcystin-LR [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
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The development of a rapid one-step antigen-immobilized inhibition ELISA for microcystin-LR is described. For microplate coating a microcystin-biotin conjugate was synthesized. Using the commercially available monoclonal antibody MC10E7 in our newly established assay, IC”5″0 values of 0.045@mgl^-^1 have been achieved. The detection limit for microcystin-LR was 4ngl^-^1. Considering the guidelines proposed by the world health organization (WHO) for microcystin-LR in drinking water (1@mgl^-^1) the sensitivity of our test is more than sufficient. The period of assay processing could successfully be shortened to about 3h without any loss in sensitivity. The suitability of the newly developed assay was evaluated with microcystin-LR spiked environmental water samples. Recovery rates for microcystin-LR between 60 and 165% were obtained in the linear range of the test format. The antigen-immobilized test format provides a highly reproducible, easy, and fast to perform detection system for microcystin allowing an internal retrospective quality control of the assay.
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ELISA
Determination of Chlorpyriphos methyl in wheat samples by enzyme linked immunosorbent assay (ELISA)
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ELISA
While this video follows a specific protocol that includes an ELISA, watching this video may provide some insight about how such an assay works or how to carry out one. Please leave any comments if this was useful or if it needs work.
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Elisa Assay Questions
Question by allaboutbaby: ELISA comes under anyof the following assays? i.e, cell based assay, binding assay,biochemical assay?
Best answer:
Answer by jonmcn49ELISA is an acronym for; ” Enzyme linked immunosorbent assay. ” Try binding, as it is bound to its enzyme and then bound by its antibody to an epitope of interest.
Add your own answer in the [...]
Question by ntinatsepa: ELISA (Enzyme Linked Immuno Sorbent Assay?
blood test: ELISA (Enzyme Linked Immuno Sorbent Assay
what do you know about it?
Best answer:
Answer by hcbiochemWhat do you want or need to know about them?
What do you think? Answer below!
Question by teck kim: in ELISA, why the assay need secondary antibody labeled, why no just label the primary antibody?
Best answer:
Answer by Isopropyl_Dogantibodies have two sides: a constant region and a variable region. So the primary antibody has a variable region that specifically binds to the antigen of interest… this primary antibody can be made [...]