Archive for June, 2010
Last Updated on Wednesday, 30 June 2010 09:14 Written by Administrator Wednesday, 30 June 2010 09:14
www.rapidtest.com Cardiac Markers Elisa kits – Cardiac Markers Elisa assays: DIGOXIN ELISA kit ELISA kits manufacturer – ElLISA assays supplier: Diagnostic Automation/Cortez Diagnostics, Inc., Tel: 818-591 3030, USA.
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Last Updated on Wednesday, 30 June 2010 01:15 Written by Administrator Wednesday, 30 June 2010 01:15
An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5ngmL^-^1 of bacopaside I and the limit of detection was 0.1ngmL^-^1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.
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Tags: Acta, against, Analytica, antibodies, article, assay, bacopaside, Chimica, EnzymeLinked, from, IMMUNOSORBANT, polyclonal, using | Posted under Elisa Assay Books | No Comments
Last Updated on Monday, 28 June 2010 05:23 Written by Administrator Monday, 28 June 2010 05:23
Plastic enzyme-linked immunosorbent assays (ELISA)-on-a-chip biosensor for botulinum neurotoxin A [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A plastic ELISA-on-a-chip (EOC) employing the concept of cross-flow immuno-chromatographic analysis was applied to the measurement of botulinum neurotoxin A (BoNT/A) as agent for bio-terrorism. Two monoclonal antibodies specific to the heavy chain of the toxin were raised and identified to form sandwich binding complexes as the pair with the analyte. For the construction of an immuno-strip, one was utilized as the capture antibody immobilized onto nitrocellulose membrane and the other as the detection coupled to an enzyme, horseradish peroxidase. The two plates of EOC used in this study were fabricated by injection molding of polycarbonate to improve the reproducibility of manufacture and, after inclusion of the immuno-strip, bonded using a UV-sensitive adhesive. Under optimal conditions of analysis, the chip produced a color signal in proportion to the analyte dose and the signal was quantified using a detector equipped with a digital camera. From the dose-response curve, the detection limit of BoNT/A was 2.0ngmL^-^1, approximately five times more sensitive than a commercial-version detection kit employing colloidal gold tracer.
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Tags: Acta, Analytica, article, assays, biosensor, botulinum, Chimica, ELISAonachip, EnzymeLinked, from, Immunosorbent, neurotoxin, Plastic | Posted under Elisa Assay Books | No Comments
Last Updated on Monday, 28 June 2010 02:20 Written by Administrator Monday, 28 June 2010 02:20
www.rapidtest.com Cardiac Markers Elisa kits – Cardiac Markers Elisa assays: ELISA Test KIt for Quantitative Measurement of Creatine-Kinase (CK-MB), Human CK-MB ELISA Test Kit. ELISA kits manufacturer – ElLISA assays supplier: Diagnostic Automation/Cortez Diagnostics, Inc., Tel: 818-591 3030, USA
Tags: CKMB, CREATINE, elisa, Enzyme, Human, immunoassay, KINASE, test | Posted under Elisa Assay Videos | No Comments