Development of a class selective immunoassay for the type II pyrethroid insecticides [An article from: Analytica Chimica Acta]
Development of a class selective immunoassay for the type II pyrethroid insecticides [An article from: Analytica Chimica Acta]
Last Updated on Wednesday, 16 June 2010 08:32 Written by Administrator Wednesday, 16 June 2010 08:32
Development of a class selective immunoassay for the type II pyrethroid insecticides [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A general and broad class selective enzyme-linked immunosorbent assay was developed for the type II pyrethroid insecticides, such as cypermethrin, deltamethrin, cyhalothrin, cyfluthrin, fenvalerate, esfenvalerate and fluvalinate. Polyclonal antibodies were generated by immunizing with a type II pyrethroid immunogen ((RS)-@a-cyano-3-phenoxybenzyl (RS)-cis,trans-2,2-dimethyl-3-carboxyl-cyclopropanecarboxylate) conjugated with thyroglobulin. Antisera were screened against nine different coating antigens. The antibody-antigen combination with the most selectivity for type II pyrethroids such as cypermethrin was further optimized and tested for tolerance to co-solvent, pH and ionic strength changes. The IC”5″0s of the optimized immunoassay were 78@mgl^-^1 for cypermethrin, 205@mgl^-^1 for cyfluthrin, 120@mgl^-^1 for cyhalothrin, 13@mgl^-^1 for deltamethrin, 6@mgl^-^1 for esfenvalerate, 8@mgl^-^1 for fenvalerate and 123@mgl^-^1 for fluvalinate. No cross-reactivity was measured for the type I pyrethroids such as permethrin, bifenthrin, phenothrin, resmethrin and bioresmethrin. This assay can be used in monitoring studies to distinguish between type I and II pyrethroids.
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