In IFN-g assay, why we collect supernatants of spleen cells after 72 hrs of incubation with antigens?

Question by vijay_hotguy: In IFN-g assay, why we collect supernatants of spleen cells after 72 hrs of incubation with antigens?
For IL-2 bioassay using CTLL cell lines, we use supernatants of splenocytes after 24 hrs of incubation with antigens and for IFN-g Elisa assay it was collected after 72 hrs of incubation. can you explain what might be the reason? Thanks in advance.

Best answer:

Answer by NeuroProf
Yes, IFN-gamma is produced by splenic cells in reaction to infection or allergy. So essentially what you are doing is fooling the cultured cells into thinking there is a major immune response required, and thus they generate IFN-gamma

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