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Plastic enzyme-linked immunosorbent assays (ELISA)-on-a-chip biosensor for botulinum neurotoxin A [An article from: Analytica Chimica Acta]
Plastic enzyme-linked immunosorbent assays (ELISA)-on-a-chip biosensor for botulinum neurotoxin A [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A plastic ELISA-on-a-chip (EOC) employing the concept of cross-flow immuno-chromatographic analysis was applied to the measurement of botulinum neurotoxin A (BoNT/A) as agent for bio-terrorism. Two monoclonal antibodies specific to the heavy chain of the toxin were raised and identified to form sandwich binding complexes as the pair with the analyte. For the construction of an immuno-strip, one was utilized as the capture antibody immobilized onto nitrocellulose membrane and the other as the detection coupled to an enzyme, horseradish peroxidase. The two plates of EOC used in this study were fabricated by injection molding of polycarbonate to improve the reproducibility of manufacture and, after inclusion of the immuno-strip, bonded using a UV-sensitive adhesive. Under optimal conditions of analysis, the chip produced a color signal in proportion to the analyte dose and the signal was quantified using a detector equipped with a digital camera. From the dose-response curve, the detection limit of BoNT/A was 2.0ngmL^-^1, approximately five times more sensitive than a commercial-version detection kit employing colloidal gold tracer.
List Price: $ 10.95
Price: $ 10.95
Human CK-MB ELISA Kit, CREATINE KINASE (CK-MB) ENZYME IMMUNOASSAY TEST KIT
www.rapidtest.com Cardiac Markers Elisa kits – Cardiac Markers Elisa assays: ELISA Test KIt for Quantitative Measurement of Creatine-Kinase (CK-MB), Human CK-MB ELISA Test Kit. ELISA kits manufacturer – ElLISA assays supplier: Diagnostic Automation/Cortez Diagnostics, Inc., Tel: 818-591 3030, USA
Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion [An article from: Analytica Chimica Acta]
Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Sensitive detection of pesticides is of utmost importance in environment and food analysis. Immunological methods are widely used to detect pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952 microcontroller for precise flow of reagents, samples, substrate and conjugates used for analysis to be passed through an immobilized antibody column at predetermined time. With the sequence and flow control of buffers used, it also provides the option for reuse of the immobilized antibody column. The system is flexible to accommodate multiple sequences up to a maximum of 99 steps. It is customizable for different flow ELISA applications. It can control up to eight solenoid valves (dc 24V) and two peristaltic pumps and has one 12bit analog channel for data acquisition. With the serial interface port, the system provides convenient means for data acquisition into the computer. The system has been successfully tested for immuno analysis of organophosphorous pesticide methyl parathion.
List Price: $ 8.95
Price: $ 8.95
Determination of glycyrrhizin in Chinese prescriptions and biological samples by enzyme-linked immunosorbent assay [An article from: Analytica Chimica Acta]
Determination of glycyrrhizin in Chinese prescriptions and biological samples by enzyme-linked immunosorbent assay [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
In the work described on this paper, a highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) for the determination of glycyrrhizin (GL) was developed. To demonstrate the potential of the ELISA, a preliminary pharmacokinetic study of GL in rats and quantitative analysis of GL in several Chinese prescriptions were performed and compared with high-performance liquid chromatography (HPLC). The experimental data indicated that ELISA method exhibits more advantages over HPLC, such as lower detection limit, high specificity, low background and no requirement of sample pre-treatment, and more suitable for the determination of natural components in Chinese traditional medicines.
List Price: $ 10.95
Price: $ 10.95
Elisa Assay Questions
Question by allaboutbaby: ELISA comes under anyof the following assays? i.e, cell based assay, binding assay,biochemical assay?
Best answer:
Answer by jonmcn49ELISA is an acronym for; ” Enzyme linked immunosorbent assay. ” Try binding, as it is bound to its enzyme and then bound by its antibody to an epitope of interest.
Add your own answer in the [...]
Question by ntinatsepa: ELISA (Enzyme Linked Immuno Sorbent Assay?
blood test: ELISA (Enzyme Linked Immuno Sorbent Assay
what do you know about it?
Best answer:
Answer by hcbiochemWhat do you want or need to know about them?
What do you think? Answer below!
Question by teck kim: in ELISA, why the assay need secondary antibody labeled, why no just label the primary antibody?
Best answer:
Answer by Isopropyl_Dogantibodies have two sides: a constant region and a variable region. So the primary antibody has a variable region that specifically binds to the antigen of interest… this primary antibody can be made [...]