Posts Tagged ‘Acta’
Last Updated on Saturday, 10 July 2010 04:04 Written by Administrator Saturday, 10 July 2010 04:04
Development of a highly sensitive inhibition immunoassay for microcystin-LR [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
The development of a rapid one-step antigen-immobilized inhibition ELISA for microcystin-LR is described. For microplate coating a microcystin-biotin conjugate was synthesized. Using the commercially available monoclonal antibody MC10E7 in our newly established assay, IC”5″0 values of 0.045@mgl^-^1 have been achieved. The detection limit for microcystin-LR was 4ngl^-^1. Considering the guidelines proposed by the world health organization (WHO) for microcystin-LR in drinking water (1@mgl^-^1) the sensitivity of our test is more than sufficient. The period of assay processing could successfully be shortened to about 3h without any loss in sensitivity. The suitability of the newly developed assay was evaluated with microcystin-LR spiked environmental water samples. Recovery rates for microcystin-LR between 60 and 165% were obtained in the linear range of the test format. The antigen-immobilized test format provides a highly reproducible, easy, and fast to perform detection system for microcystin allowing an internal retrospective quality control of the assay.
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Tags: Acta, Analytica, article, Chimica, Development, from, highly, immunoassay, inhibition, microcystinLR, sensitive | Posted under Elisa Assay Books | No Comments
Last Updated on Wednesday, 30 June 2010 01:15 Written by Administrator Wednesday, 30 June 2010 01:15
An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5ngmL^-^1 of bacopaside I and the limit of detection was 0.1ngmL^-^1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.
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Tags: Acta, against, Analytica, antibodies, article, assay, bacopaside, Chimica, EnzymeLinked, from, IMMUNOSORBANT, polyclonal, using | Posted under Elisa Assay Books | No Comments
Last Updated on Monday, 28 June 2010 05:23 Written by Administrator Monday, 28 June 2010 05:23
Plastic enzyme-linked immunosorbent assays (ELISA)-on-a-chip biosensor for botulinum neurotoxin A [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A plastic ELISA-on-a-chip (EOC) employing the concept of cross-flow immuno-chromatographic analysis was applied to the measurement of botulinum neurotoxin A (BoNT/A) as agent for bio-terrorism. Two monoclonal antibodies specific to the heavy chain of the toxin were raised and identified to form sandwich binding complexes as the pair with the analyte. For the construction of an immuno-strip, one was utilized as the capture antibody immobilized onto nitrocellulose membrane and the other as the detection coupled to an enzyme, horseradish peroxidase. The two plates of EOC used in this study were fabricated by injection molding of polycarbonate to improve the reproducibility of manufacture and, after inclusion of the immuno-strip, bonded using a UV-sensitive adhesive. Under optimal conditions of analysis, the chip produced a color signal in proportion to the analyte dose and the signal was quantified using a detector equipped with a digital camera. From the dose-response curve, the detection limit of BoNT/A was 2.0ngmL^-^1, approximately five times more sensitive than a commercial-version detection kit employing colloidal gold tracer.
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Tags: Acta, Analytica, article, assays, biosensor, botulinum, Chimica, ELISAonachip, EnzymeLinked, from, Immunosorbent, neurotoxin, Plastic | Posted under Elisa Assay Books | No Comments
Last Updated on Saturday, 26 June 2010 12:50 Written by Administrator Saturday, 26 June 2010 12:50
Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Sensitive detection of pesticides is of utmost importance in environment and food analysis. Immunological methods are widely used to detect pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952 microcontroller for precise flow of reagents, samples, substrate and conjugates used for analysis to be passed through an immobilized antibody column at predetermined time. With the sequence and flow control of buffers used, it also provides the option for reuse of the immobilized antibody column. The system is flexible to accommodate multiple sequences up to a maximum of 99 steps. It is customizable for different flow ELISA applications. It can control up to eight solenoid valves (dc 24V) and two peristaltic pumps and has one 12bit analog channel for data acquisition. With the serial interface port, the system provides convenient means for data acquisition into the computer. The system has been successfully tested for immuno analysis of organophosphorous pesticide methyl parathion.
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Tags: Acta, analysis, Analytica, article, assay, Automated, Chimica, elisa, EnzymeLinked, flow, from, Immunosorbent, methyl, parathion, system | Posted under Elisa Assay Books | No Comments