Posts Tagged ‘antigens’
Last Updated on Friday, 25 June 2010 03:25 Written by Administrator Friday, 25 June 2010 03:25
Question by vijay_hotguy: In IFN-g assay, why we collect supernatants of spleen cells after 72 hrs of incubation with antigens?
For IL-2 bioassay using CTLL cell lines, we use supernatants of splenocytes after 24 hrs of incubation with antigens and for IFN-g Elisa assay it was collected after 72 hrs of incubation. can you explain what might be the reason? Thanks in advance.
Answer by NeuroProf
Yes, IFN-gamma is produced by splenic cells in reaction to infection or allergy. So essentially what you are doing is fooling the cultured cells into thinking there is a major immune response required, and thus they generate IFN-gamma
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Last Updated on Thursday, 24 June 2010 04:14 Written by Administrator Thursday, 24 June 2010 04:14
Rapid, quantitative Tube immunoassays for on-site detection of Botrytis, Aspergillus and Penicillium antigens in grape juice [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Two Tube immunoassays have been developed for the detection and quantification of fungal antigens in grape juice. One for Botrytis, the main cause of Bunch rot in grapes in California, and the other for Botrytis plus rot caused by species of Aspergillus and Penicillium, fungi that can also be involved in Bunch rot. The assays, which are rapid, user friendly and do not require laboratory facilities were designed and tested for on-site use at wineries during harvest. Both assays employ highly specific monoclonal antibodies, BC12.CA4, for Botrytis and an hitherto unreported antibody, AF-CA2, for all species of Aspergillus and Penicillium. The protocol for both assays is the same and mirrors that used in plate-trapped antigen (PTA) ELISAs. The antibodies detect water-soluble fungal antigens present in juice from infected grapes that bind rapidly to the walls of Nunc immunosorbent tubes. Saline extracts from freeze-dried mycelial cultures of Botrytis cinerea and Aspergillus niger are used as standards. The assays are very sensitive detecting between 1 and 3% levels of rot in grapes corresponding to 10-30@mg/ml extracts from freeze-dried mycelium. Absorbance values from the Tube assays parallel but do not equal those from PTA-ELISA tests. No problems were encountered with tests carried out on the testing stands at wineries at harvest time by personnel with no scientific background.
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