Posts Tagged ‘assay’
Last Updated on Saturday, 10 July 2010 06:07 Written by Administrator Saturday, 10 July 2010 06:07
Question by allaboutbaby: ELISA comes under anyof the following assays? i.e, cell based assay, binding assay,biochemical assay?
Best answer:
Answer by jonmcn49
ELISA is an acronym for; ” Enzyme linked immunosorbent assay. ” Try binding, as it is bound to its enzyme and then bound by its antibody to an epitope of interest.
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Tags: anyof, assay, assaybiochemical, assays, based, binding, cell, comes, elisa, following, under | Posted under Elisa Assay Questions | No Comments
Last Updated on Sunday, 4 July 2010 10:48 Written by Administrator Sunday, 4 July 2010 10:48
Question by ntinatsepa: ELISA (Enzyme Linked Immuno Sorbent Assay?
blood test: ELISA (Enzyme Linked Immuno Sorbent Assay
what do you know about it?
Best answer:
Answer by hcbiochem
What do you want or need to know about them?
What do you think? Answer below!
Tags: assay, elisa, Enzyme, Immuno, Linked, Sorbent | Posted under Elisa Assay Questions | 4 Comments
Last Updated on Friday, 2 July 2010 10:09 Written by Administrator Friday, 2 July 2010 10:09
Question by teck kim: in ELISA, why the assay need secondary antibody labeled, why no just label the primary antibody?
Best answer:
Answer by Isopropyl_Dog
antibodies have two sides: a constant region and a variable region. So the primary antibody has a variable region that specifically binds to the antigen of interest… this primary antibody can be made naturally by the immune system of an organism. If a person is exposed to antigen X, the natural process in his body will create anti-X antibodies. So then we could take a sample of his blood and collect the anti-X antibodies (with affinity chromatography this is easy), purify them and use them in an ELISA to detect antigen X. Unfortunately the natural process does not label the antibodies… thats why we need secondary antibodies.
The secondary antibody specifically binds to the constant region of the primary antibody (all the primary antibodies will have similar constant regions) and has a detectable probe. So you can use the same secondary antibody with lots of different primary antibodies.
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Last Updated on Wednesday, 30 June 2010 01:15 Written by Administrator Wednesday, 30 June 2010 01:15
An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I-BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95-62.5ngmL^-^1 of bacopaside I and the limit of detection was 0.1ngmL^-^1. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.
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Tags: Acta, against, Analytica, antibodies, article, assay, bacopaside, Chimica, EnzymeLinked, from, IMMUNOSORBANT, polyclonal, using | Posted under Elisa Assay Books | No Comments