Posts Tagged ‘assay’
Last Updated on Saturday, 26 June 2010 12:50 Written by Administrator Saturday, 26 June 2010 12:50
Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Sensitive detection of pesticides is of utmost importance in environment and food analysis. Immunological methods are widely used to detect pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952 microcontroller for precise flow of reagents, samples, substrate and conjugates used for analysis to be passed through an immobilized antibody column at predetermined time. With the sequence and flow control of buffers used, it also provides the option for reuse of the immobilized antibody column. The system is flexible to accommodate multiple sequences up to a maximum of 99 steps. It is customizable for different flow ELISA applications. It can control up to eight solenoid valves (dc 24V) and two peristaltic pumps and has one 12bit analog channel for data acquisition. With the serial interface port, the system provides convenient means for data acquisition into the computer. The system has been successfully tested for immuno analysis of organophosphorous pesticide methyl parathion.
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Tags: Acta, analysis, Analytica, article, assay, Automated, Chimica, elisa, EnzymeLinked, flow, from, Immunosorbent, methyl, parathion, system | Posted under Elisa Assay Books | No Comments
Last Updated on Friday, 25 June 2010 04:08 Written by Administrator Friday, 25 June 2010 04:08
Determination of glycyrrhizin in Chinese prescriptions and biological samples by enzyme-linked immunosorbent assay [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
In the work described on this paper, a highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) for the determination of glycyrrhizin (GL) was developed. To demonstrate the potential of the ELISA, a preliminary pharmacokinetic study of GL in rats and quantitative analysis of GL in several Chinese prescriptions were performed and compared with high-performance liquid chromatography (HPLC). The experimental data indicated that ELISA method exhibits more advantages over HPLC, such as lower detection limit, high specificity, low background and no requirement of sample pre-treatment, and more suitable for the determination of natural components in Chinese traditional medicines.
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Tags: Acta, Analytica, article, assay, biological, Chimica, Chinese, determination, EnzymeLinked, from, glycyrrhizin, Immunosorbent, prescriptions, samples | Posted under Elisa Assay Books | No Comments
Last Updated on Friday, 25 June 2010 03:25 Written by Administrator Friday, 25 June 2010 03:25
Question by vijay_hotguy: In IFN-g assay, why we collect supernatants of spleen cells after 72 hrs of incubation with antigens?
For IL-2 bioassay using CTLL cell lines, we use supernatants of splenocytes after 24 hrs of incubation with antigens and for IFN-g Elisa assay it was collected after 72 hrs of incubation. can you explain what might be the reason? Thanks in advance.
Best answer:
Answer by NeuroProf
Yes, IFN-gamma is produced by splenic cells in reaction to infection or allergy. So essentially what you are doing is fooling the cultured cells into thinking there is a major immune response required, and thus they generate IFN-gamma
Add your own answer in the comments!
Tags: after, antigens, assay, cells, collect, IFNg, incubation, spleen, supernatants | Posted under Elisa Assay Questions | No Comments
ENZYME-LINKED IMMUNOSORBANT ASSAY (ELISA): An entry from Gale’s World of Microbiology and Immunology
Last Updated on Thursday, 24 June 2010 02:55 Written by Administrator Thursday, 24 June 2010 02:55
ENZYME-LINKED IMMUNOSORBANT ASSAY (ELISA): An entry from Gale’s World of Microbiology and Immunology
This digital document is an article from World of Microbiology and Immunology, brought to you by Gale®, a part of Cengage Learning, a world leader in e-research and educational publishing for libraries, schools and businesses. The length of the article is 493 words. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser. Covers the concepts, theories, discoveries, and pioneers in microbiology and immunology, using a mix of traditional academic and topical articles, this title addresses current ethical, legal, and social issues with special emphasis given to biological warfare and terrorism.
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Tags: assay, elisa, entry, EnzymeLinked, from, Gale's, Immunology, IMMUNOSORBANT, Microbiology, World | Posted under Elisa Assay Books | No Comments