Posts Tagged ‘Development’
Last Updated on Saturday, 10 July 2010 04:04 Written by Administrator Saturday, 10 July 2010 04:04
Development of a highly sensitive inhibition immunoassay for microcystin-LR [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
The development of a rapid one-step antigen-immobilized inhibition ELISA for microcystin-LR is described. For microplate coating a microcystin-biotin conjugate was synthesized. Using the commercially available monoclonal antibody MC10E7 in our newly established assay, IC”5″0 values of 0.045@mgl^-^1 have been achieved. The detection limit for microcystin-LR was 4ngl^-^1. Considering the guidelines proposed by the world health organization (WHO) for microcystin-LR in drinking water (1@mgl^-^1) the sensitivity of our test is more than sufficient. The period of assay processing could successfully be shortened to about 3h without any loss in sensitivity. The suitability of the newly developed assay was evaluated with microcystin-LR spiked environmental water samples. Recovery rates for microcystin-LR between 60 and 165% were obtained in the linear range of the test format. The antigen-immobilized test format provides a highly reproducible, easy, and fast to perform detection system for microcystin allowing an internal retrospective quality control of the assay.
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Last Updated on Wednesday, 16 June 2010 08:32 Written by Administrator Wednesday, 16 June 2010 08:32
Development of a class selective immunoassay for the type II pyrethroid insecticides [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
A general and broad class selective enzyme-linked immunosorbent assay was developed for the type II pyrethroid insecticides, such as cypermethrin, deltamethrin, cyhalothrin, cyfluthrin, fenvalerate, esfenvalerate and fluvalinate. Polyclonal antibodies were generated by immunizing with a type II pyrethroid immunogen ((RS)-@a-cyano-3-phenoxybenzyl (RS)-cis,trans-2,2-dimethyl-3-carboxyl-cyclopropanecarboxylate) conjugated with thyroglobulin. Antisera were screened against nine different coating antigens. The antibody-antigen combination with the most selectivity for type II pyrethroids such as cypermethrin was further optimized and tested for tolerance to co-solvent, pH and ionic strength changes. The IC”5″0s of the optimized immunoassay were 78@mgl^-^1 for cypermethrin, 205@mgl^-^1 for cyfluthrin, 120@mgl^-^1 for cyhalothrin, 13@mgl^-^1 for deltamethrin, 6@mgl^-^1 for esfenvalerate, 8@mgl^-^1 for fenvalerate and 123@mgl^-^1 for fluvalinate. No cross-reactivity was measured for the type I pyrethroids such as permethrin, bifenthrin, phenothrin, resmethrin and bioresmethrin. This assay can be used in monitoring studies to distinguish between type I and II pyrethroids.
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