Posts Tagged ‘elisa’
Last Updated on Sunday, 4 July 2010 07:48 Written by Administrator Sunday, 4 July 2010 07:48
www.rapidtest.com Cardiac Markers Elisa kits – Cardiac Markers Elisa assays: ELISA Test KIt for Quantitative Measurement of Creatine-Kinase (CK-MB), Human CK-MB ELISA Test Kit. ELISA kits manufacturer – ElLISA assays supplier: Diagnostic Automation/Cortez Diagnostics, Inc., Tel: 818-591 3030, USA
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Tags: CKMB, elisa | Posted under Elisa Assay Videos | No Comments
Last Updated on Sunday, 4 July 2010 10:48 Written by Administrator Sunday, 4 July 2010 10:48
Question by ntinatsepa: ELISA (Enzyme Linked Immuno Sorbent Assay?
blood test: ELISA (Enzyme Linked Immuno Sorbent Assay
what do you know about it?
Best answer:
Answer by hcbiochem
What do you want or need to know about them?
What do you think? Answer below!
Tags: assay, elisa, Enzyme, Immuno, Linked, Sorbent | Posted under Elisa Assay Questions | 4 Comments
Last Updated on Friday, 2 July 2010 10:09 Written by Administrator Friday, 2 July 2010 10:09
Question by teck kim: in ELISA, why the assay need secondary antibody labeled, why no just label the primary antibody?
Best answer:
Answer by Isopropyl_Dog
antibodies have two sides: a constant region and a variable region. So the primary antibody has a variable region that specifically binds to the antigen of interest… this primary antibody can be made naturally by the immune system of an organism. If a person is exposed to antigen X, the natural process in his body will create anti-X antibodies. So then we could take a sample of his blood and collect the anti-X antibodies (with affinity chromatography this is easy), purify them and use them in an ELISA to detect antigen X. Unfortunately the natural process does not label the antibodies… thats why we need secondary antibodies.
The secondary antibody specifically binds to the constant region of the primary antibody (all the primary antibodies will have similar constant regions) and has a detectable probe. So you can use the same secondary antibody with lots of different primary antibodies.
Know better? Leave your own answer in the comments!
Last Updated on Wednesday, 30 June 2010 09:14 Written by Administrator Wednesday, 30 June 2010 09:14
www.rapidtest.com Cardiac Markers Elisa kits – Cardiac Markers Elisa assays: DIGOXIN ELISA kit ELISA kits manufacturer – ElLISA assays supplier: Diagnostic Automation/Cortez Diagnostics, Inc., Tel: 818-591 3030, USA.
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