Posts Tagged ‘flow’
Last Updated on Saturday, 26 June 2010 12:50 Written by Administrator Saturday, 26 June 2010 12:50
Automated flow enzyme-linked immunosorbent assay (ELISA) system for analysis of methyl parathion [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Sensitive detection of pesticides is of utmost importance in environment and food analysis. Immunological methods are widely used to detect pesticides in agricultural and environmental samples wherein antibodies are employed against the target molecules. Accurate diagnosis depends on the affinity and specificity of the antibody preparation used, and high affinity antibodies are essential for the detection of very small amounts of pesticides. Enzyme linked immuno sorbent assay (ELISA) coupled with flow injection analysis (FIA) technique provides a very high sensitivity with high throughput of analyses. Automation of this analysis scheme ensures precise detection with high accuracy. The present development aims at providing a user-friendly system for achieving this objective. It employs a 8952 microcontroller for precise flow of reagents, samples, substrate and conjugates used for analysis to be passed through an immobilized antibody column at predetermined time. With the sequence and flow control of buffers used, it also provides the option for reuse of the immobilized antibody column. The system is flexible to accommodate multiple sequences up to a maximum of 99 steps. It is customizable for different flow ELISA applications. It can control up to eight solenoid valves (dc 24V) and two peristaltic pumps and has one 12bit analog channel for data acquisition. With the serial interface port, the system provides convenient means for data acquisition into the computer. The system has been successfully tested for immuno analysis of organophosphorous pesticide methyl parathion.
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Price: $ 8.95
Tags: Acta, analysis, Analytica, article, assay, Automated, Chimica, elisa, EnzymeLinked, flow, from, Immunosorbent, methyl, parathion, system | Posted under Elisa Assay Books | No Comments
Last Updated on Wednesday, 23 June 2010 06:27 Written by Administrator Wednesday, 23 June 2010 06:27
Automated flow fluorescent immunoassay for part per trillion detection of the neonicotinoid insecticide thiamethoxam [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
An ultra sensitive automated flow fluorescent immunoassay was developed using the KinExA(TM) 3000 system for quantitative analysis of the neonicotinoid insecticide thiamethoxam. Five new monoclonal antibodies were obtained and screened with a competitive ELISA. One monoclonal antibody designated as E6VI was evaluated for sensitivity, selectivity and solvent tolerance with the KinExA. Sensitivity determined from the concentration of half-maximal inhibition (IC”5″0) was obtained by plotting KinExA signals to a four-parameter sigmoidal curve as a function of analyte concentrations. For the most sensitive clone, the IC”5″0 and the limit of detection were approximately 30pgml^-^1 and 16pgml^-^1, respectively. Cross-reactivity was estimated by measuring the equilibrium constants (K”d) for four other neonicotinoid insecticides (clothianidin, imidacloprid, dinotefuran, and acetamiprid). E6VI was very specific to thiamethoxam with <0.11% cross-reactivity for tested neonicotinoids. An excellent correlation (r^2=0.99) was obtained between spiked and measured concentrations of thiamethoxam in stream and tap water, potato, cucumber, and apple samples.
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Price: $ 10.95
Tags: Acta, Analytica, article, Automated, Chimica, detection, flow, fluorescent, from, immunoassay, insecticide, neonicotinoid, part, thiamethoxam, trillion | Posted under Elisa Assay Books | No Comments