Posts Tagged ‘thiamethoxam’
Last Updated on Wednesday, 23 June 2010 06:27 Written by Administrator Wednesday, 23 June 2010 06:27
Automated flow fluorescent immunoassay for part per trillion detection of the neonicotinoid insecticide thiamethoxam [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
An ultra sensitive automated flow fluorescent immunoassay was developed using the KinExA(TM) 3000 system for quantitative analysis of the neonicotinoid insecticide thiamethoxam. Five new monoclonal antibodies were obtained and screened with a competitive ELISA. One monoclonal antibody designated as E6VI was evaluated for sensitivity, selectivity and solvent tolerance with the KinExA. Sensitivity determined from the concentration of half-maximal inhibition (IC”5″0) was obtained by plotting KinExA signals to a four-parameter sigmoidal curve as a function of analyte concentrations. For the most sensitive clone, the IC”5″0 and the limit of detection were approximately 30pgml^-^1 and 16pgml^-^1, respectively. Cross-reactivity was estimated by measuring the equilibrium constants (K”d) for four other neonicotinoid insecticides (clothianidin, imidacloprid, dinotefuran, and acetamiprid). E6VI was very specific to thiamethoxam with <0.11% cross-reactivity for tested neonicotinoids. An excellent correlation (r^2=0.99) was obtained between spiked and measured concentrations of thiamethoxam in stream and tap water, potato, cucumber, and apple samples.
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